METHODS Cytotoxicity assay was tested by MTT method. The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry, AO/ EB double fluorescence stain and terminal deoxynucleotidyl transferase mediated nick end labeling ( TUNEL). 方法采用MTT比色法检测药物的细胞毒性作用,并用流式细胞术、AO/EB双重荧光染色法和TUNEL法测定甲基莲心碱对长春新碱诱导人胃癌细胞凋亡率的影响。
The thesis makes an exploratory research on the interface slip of the double steel-concrete composite beam under negative bending with the primary mechanical principle and the boundary method of stain distributions respectively. 本文分别运用基本力学方法和应变分布边界方法对钢&混凝土双面结合梁的滑移效应进行了探索性分析。
Conclusions: Ponceau 2R-brilliant green double staining technique is a quick, simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system. 结论:丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。
The significance of shoulder force statistical measurement is explained, and a sys-tem with double-shear beam stain gage force transducer for measuring the shoulder-force is introduced. 介绍了立姿射击的抵肩力统计测量的意义及采用双剪切梁式应变测力传感器测量抵肩力的系统组成;
Methods: The spinal cord sections of the adult Sprague-Dawley rats were detected by using Ponceau 2R-brilliant green double stain method. 方法:应用丽春红2R-亮绿双重染色法,对正常SD大鼠脊髓组织进行染色。
Action Analysis of Cholinesterase and Silver-plating Double Histochemical Stain of the Skeletal Muscles in Rats 大鼠骨骼肌胆碱酯酶镀银双重组化染色的作用分析
The double fluorescence stain with acridine orange and ethidium bromide was performed to detect the cell vitality. 采用吖啶橙、嗅乙啶双荧光染色观察细胞活率;
The β Glc was localized by double cell stain experiment. 细胞双染法确定βGlc在细胞内的定位。